Purification and characterization of bacteriocin isolated from Streptococcus thermophilus

Purification and Characterization of Bacteriocin Produced by Streptococcus Phocae Pi80

Purification and certain properties of a bacteriocin from Streptococcus mutans.

An inhibition factor from Streptococcus mutans strain C3603 (serotype c) was purified and isolated, and its properties indicated that it was a bacteriocin. Bacteriocin C3603 is a basic protein with a pI value of 10 and a molecular weight of 4,800. The activity of this bacteriocin was not affected by pH over a range of 1.0 to 12.0 or by storage at 100 degrees C for 10 min at pH 2.0 to 7.0 or sto...

Cloning and characterization of the galactokinase gene from Streptococcus thermophilus.

The objective of this research was to clone and characterize the galactokinase gene (galK) from Streptococcus thermophilus F410. Partially digested genomic DNA was cloned into pBR322 and transformed into galK Escherichia coli, and a galactose-fermenting transformant was isolated. Restriction analysis revealed that the transformant resulted from a Sau3A-HindIII 4.0-kb fragment. Galactokinase act...

Probiotic Characterization of Lactic Acid Bacteria Isolated from Fermented Foods and Partial Purification of Its Bacteriocin

The present research was focused on probiotic characterization of lactic acid bacteria from fermented foods and beverage of Kinnaur and partial purification of bacteriocin. Lactic acid bacterial isolates were examined in-vitro for potential probiotic properties based on their low pH tolerance, bile-salt resistance, production of antimicrobial substances, exopolysaccharide production, proteolyti...

Purification and Characterization of a Bacteriocin Produced by Lactobacillus lactis Isolated from Marine Environment

Bacteriocin producing Lactobacillus lactis strain isolated from marine environment, showed broad range of antibacterial activity against some major food borne pathogens. Maximum bacteriocin production was observed at 30°C , pH 6.0 and 1.5% sodium chloride so lution. In addition of enzymes, "-amylase, DNase, RNase and lipase were slightly positive effect bacteriocin production. Proteinase K and ...